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5-Aminoallyluridine-5'-Triphosphate (AA-UTP)Order

Features: ideal for indirect non-radioactive enzymatic labeling of RNA during in vitro transcription.


Formula:
C12H20N3O15P3 (acid form)
Molecular weight: 539.2 (acid form)
Concentration 0.1 M

Description

Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases. The resulting amine-containing RNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin, or hapten. Dye labeled aminoallyl modified RNAs are useful in microarray analysis and have been used for localization of RNA in cell. Also it is possible to couple 1,10-phenanthroline to an aminoallyl modified RNA for sequence specific cleavage of nucleic acids.
Since AA-UTP (and AA-CTP) maintains a strict Watson-Crick base pairing recognition it is suited for the systematic evolution of ligands by exponential enrichment (SELEX) process thus allowing an introduction of primary amino-functionalities to RNA libraries.
 


Quality Control 
  • Functionally tested in in vitro transcription with T7 RNA Polymerase
  • Greater than 96% purity confirmed by HPLC
  • Free of endo-, exodeoxyribonuclease, ribonuclease, and nicking activities
 
   
Storage              -20 C
Cat.№NameAmountPrice, €Order
N-301Aminoallyl-UTP, 100 mM10 ml1700Order
N-311Aminoallyl-UTP, 100 mM100 ml12300Order