Enzymes

The PCR products obtained with Hot Start Taq polymerase are free from unspecific products and from primer-dimers.

Concentrated up to 50 000 U/ml – by request.

Isolated from a recombinant source.
T7 polymerase is extremely promoter-specific and transcribes only DNA downstream of a T7 promoter.
Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Removal of the RNase H activity resulted in an increase of full-length cDNA products. 

Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5´ → 3´ polymerase activity, but lacks 5´ →3´ exonuclease activity. Enzyme provide strong strand displacement activity. The optimum temperature is from 60-65 ⁰C. Bst becomes heat inactivated at 80 ⁰C

TEV-protease (TEVp)