Taq DNA polymerase
Recombinant Taq DNA polymerase has 5 ?-3 ? DNA-dependent polymerase activity and 5'-3' exonuclease activity of native Taq DNA polymerase from Thermus aquaticus. The rate of Taq DNA polymerase progression depends on the complexity of the DNA template and is approximately 1.5-2 kb/min.
Catalog #
Quantity, u.a.
Qty
Taq DNA polymerase is a single polypeptide with a molecular weight of about 95 kDa. The polymerase is highly processive and can amplify fragments up to 10 thousand base pairs long. The enzyme has pronounced 5'-3' exonuclease activity and adenyltransferase activity. Transferase activity results in the addition of a single pA to both 3' ends of the double-stranded DNA obtained during amplification. The enzyme has the activity indicated on the label at 74°C and pH 8.8.
Mg2+ ions are absolutely necessary for the activity of Taq DNA polymerase. The enzyme is most active in the Mg2+ concentration range of 0.5-6 mM.
Mg2+ ions are absolutely necessary for the activity of Taq DNA polymerase. The enzyme is most active in the Mg2+ concentration range of 0.5-6 mM.
Source
Taq DNA polymerase was purified from an E. coli strain containing a plasmid with the cloned full-length Thermus aquaticus DNA polymerase gene.
Unit of activity
One unit of activity corresponds to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble DNA fraction in 30 min at 74°C. Activity measurement conditions - 50 mM Tris-HCl, pH 9.0 (at 25 °C), 50 mM KCl, 200 µM of each dNTP, including [?H]dTTP, 1.5 mM MgCl2, 0.25 mg/ml activated DNA from calf thymus.Application
DNA labeling
Storage conditions
At a temperature of -20?С.
Transport conditions
At a temperature of -20?С. Transportation at ambient temperature is permitted.
