T4 DNA ligase
T4 DNA ligase was isolated from a strain of E.coli containing a plasmid with a cloned gene of the T4 bacteriophage enzyme.
Catalog #
Quantity, u.a.
Qty
Highly processive DNA-dependent RNA polymerase from bacteriophage T7, specifically interacting with the T7 promoter and catalyzing the synthesis of RNA fragments in the 5'>3' direction on a single-stranded or double-stranded DNA template under the control of this promoter. Can incorporate modified nucleotides into the RNA chain.
Used to prepare labeled RNA probes and to carry out translation in vitro.
Unit of activity
One unit of T7 RNA polymerase activity was defined as the amount of enzyme required to incorporate 1 nmol of NTP into the acid-insoluble fraction in 60 min at 37°C.
Concentration: 400 units / ?l.
Reaction buffer
40 mM Tris-HCl pH 7.4; 15 mM MgCl2, 2 mM spermidine, 10 mM NaCl, 10 mM DTT.
Optimum temperature 37°C
Used to prepare labeled RNA probes and to carry out translation in vitro.
Unit of activity
One unit of T7 RNA polymerase activity was defined as the amount of enzyme required to incorporate 1 nmol of NTP into the acid-insoluble fraction in 60 min at 37°C.
Concentration: 400 units / ?l.
Reaction buffer
40 mM Tris-HCl pH 7.4; 15 mM MgCl2, 2 mM spermidine, 10 mM NaCl, 10 mM DTT.
Optimum temperature 37°C
Storage conditions
40 mM Tris-HCl pH 7.4; 15 mM MgCl2, 2 mM spermidine, 10 mM NaCl, 10 mM DTT, 50% glycerol. Store at -20°C. The shelf life of the enzyme is 1 year.
