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Biotin-e-aminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5'-triphosphate] (Biotin-11-dUTP)

Features: widely used compound for non-radioactive DNA labeling

Biotin-11-dUTP (Biotin-11-2’-deoxyuridine-5’-triphosphate, tetralithium salt) is a widely used compound for non-radioactive DNA labeling. Biotin-11-dUTP can be enzymatically incorporated into DNA via nick-translation, random priming, 3’-end terminal labeling or in the process of PCR. The number “11” is the number of carbon atoms in the backbone of linker between dUTP and biotin. The longer the linker is, the more effective interaction between biotin and avidin. On the other hand, the shorter the linker is, the more effective is the incorporation of dUTP into DNA.

The length of linker “11” is optimal for the majority of applications.
Formula   C 28 H 41 O 17 N 6 P 3 S ( Acid Form)
Concentration and buffer: 10 mM sodium salt solution in 10 mM Tris-HCl (pH 7.5)
Purity at least 96% (as determined by high performance ion-pair chromatography)
Storage conditions -20°C