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8-oxo-dGTP – 8-Oxo-2’-deoxyguanosine-5’-triphosphate

8-oxo-dGTP –

Molecular Formula: C10H16N5O14P3 (free acid)
Molecular Weight: 523.18 g/mol (free acid)

Purity: > 96 % (HPLC)

Concentration: 10 mM

8-Oxo-2'-deoxyguanosine-5'-Triphosphate (8-Oxo-dGTP), as well as dPTP, are considered mutagenic dNTPs which can be incorporated into DNA by PCR using standard Taq polymerase. Random mutagenesis is induced by amplification of the target in the presence of the four natural dNTPs plus the mutagenic analogs, 8-Oxo-dGTP and/or dPTP. When both mutagenic analogs are used, the rate of mutagenesis is controlled by the number of PCR cycles, where 6%, 11% and 19% mutagenesis rates are obtained after 10, 20 and 30 cycles, respectively. 8-Oxo-dGTP can mispair with A, leading to A-to-C and G-to-T transversion mutations, which occur in a 1 : 1.5 ratio in PCR. When dPTP is used in combination with 8-Oxo-dGTP in PCR, both transition mutations (A-to-G and G-to-A) and transversion mutations (A-to-C and G-to-T) can be produced.

Influence on base excision repair
Influence on erroneous incorporation by DNA-polymerases
Hydrolysis to 8-oxo-dGMP by E.-coli MutT
Triggering cell senescence through formation of ROS

Shipping: shipped on blue ice
Storage    -20 °C

For research use only!