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Biotin-15-dCTP (5-{(7-N-(6-N-(biotinyl)aminocaproyl) -amino-4-oxa-hept-1-inyl)-2’-deoxycytidyl-5’-triphosphateOrder

Features: widely used compound for non-radioactive DNA labeling


Description

 
Biotin-15-dCTP (5-{(7-N-(6-N-(biotinyl)aminocaproyl) -amino-4-oxa-hept-1-inyl)-2’-deoxycytidyl-5’-triphosphate is used for non-radioactive DNA labeling. Biotin-15-dCTP can be enzymatically incorporated into DNA via nick-translation, random priming, 3'-end terminal labeling or PCR.
 
The number "15" is the number of carbon atoms in the backbone of linker
between dCTP and biotin. The longer the linker is, the more effective interaction of biotin with
avidin occurs. From the other side, the shorter the linker is, the more effective incorporation of
dCTP into DNA can be provided.
 
The length of linker "15" is optimal for the majority of applications.
 
Purity more then 96% (ion-exchange chromatography, TLC, NMR, UV-spectroscopy).
 
Concentration 1mM.
 
Storage buffer 10mM TrisHCl, pH 7.5, 1mM EDTA.
 
Storage -20°C.
Cat.№NameAmountPrice, €Order
N-2200Biotin-15-dCTP1 ml4100Order
N-2210Biotin-15-dCTP10 ml28700Order
N-2250Biotin-15-dCTP50 ml95000Order