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Uracil-DNA Glycosylase

Features: PCR products containing dU perform as well as those containing dT when used as hybridization targets
or as templates for dideoxy sequencing.
PCR products containing dU can be cloned directly, if they are transformed into UNG–bacterial hosts.
A dU-containing substrate is readily digested by some common restriction enzymes (e.g. Eco RI and Bam HI), while others show reduced activity (e.g. Hpa I, Hind II, Hind III) on these substrates.
It is not recommended the use of dU-containing DNA for protein binding or DNA-protein interaction
E. coli uracil-DNA glycosylase catalyzes the release of free uracil from uracilcontaining DNA.
Uracil-DNA Glycosylase efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not
from oligomers (6 or less bases).

Concentration 10000 units/ml

Storage buffer 20 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM EDTA, 1 mM DTT,  0,1 mg/ml BSA, 50% glycerol

Unit definition One unit of activity is the amount of enzyme required to release of 60pmol of uracil per
minute from double-stranded, uracil-containing DNA.

Reaction buffer (10x) 200 mM TrisHCl pH8,0, 10 mM EDTA, 100 mM NaCl

Storage condition: -20oC

Source: Escherichia coli (E.coli)