Hot Start Taq DNA polymerase
The amplification product obtained using Hot Start Taq DNA polymerase is free of non-specific impurities and primer-dimers.
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Hot Start Taq DNA Polymerase is an optimized mixture of Taq DNA polymerase and anti-Taq DNA polymerase monoclonal antibodies. The antibodies block polymerase activity at room temperature (20-22°C) during preparation of the PCR reaction mixture. Inhibition of Taq DNA polymerase activity is completely removed at temperatures above 70°C. The amplification product obtained with Hot Start Taq DNA polymerase is free of non-specific impurities and primer-dimers.
Unit of activity:One unit of activity corresponds to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble DNA fraction in 30 min at 72°C.
Storage buffer:The enzyme is stored in 10 mM Tris-HCl (pH 7.0); 50 mM KCl; 0.1 mM EDTA; 50% glycerol
Reaction buffer (10x) E-3000 (purchased separately): 100 mM Tris-HCl, pH 8.5 (at 25 °C), 500 mM KCl, 0.5% (v/v) Tween 20, stabilizers Taq DNA polymerase.
Unit of activity:One unit of activity corresponds to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble DNA fraction in 30 min at 72°C.
Storage buffer:The enzyme is stored in 10 mM Tris-HCl (pH 7.0); 50 mM KCl; 0.1 mM EDTA; 50% glycerol
Reaction buffer (10x) E-3000 (purchased separately): 100 mM Tris-HCl, pH 8.5 (at 25 °C), 500 mM KCl, 0.5% (v/v) Tween 20, stabilizers Taq DNA polymerase.
Storage conditions
-20°С.
